The shopkeeper at the Sussex Sweet Shop, in Broadwater Street East, was threatened with a knife, forced upstairs and bound with tape during the robbery, which happened at around 5.30pm.
The robbers emptied the safe and stole a mobile phone before locking the shopkeeper in the bathroom and making off.
There were no customers in the shop at the time and no members of the public were hurt.
Shane Dominic Brown, 20, Hakeem Omali Baker, 19, and Adele Brissett, 22, all of no fixed address, have been charged with robbery of 3,000. All the money was recovered.
All three will appear in custody at Worthing Magistrates' Court tomorrow (Saturday May 8).
Anyone who witnessed what happened or who has information about the incident is asked to contact police on 0845 60 70 999, quoting Op Bowbridge, or the independent charity Crimestoppers anonymously on 0800 555 111.
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