There are home ties for three teams, away trips for four.
Matches are due to take place on Saturday, October 30, and winners in this round pick up a neat £2,450, with losers taking £625 each.
Here are the ties for Sussex' s teams:
Bishop’s Stortford v Lewes
Hastings United v Felixstowe & Walton United
Horsham v Carshalton Athletic
Chipstead v Whitehawk
Chertsey Town v Worthing
Bognor Regis Town v Waltham Abbey
Canvey Island v Burgess Hill Town
* Eastbourne Borough of National South will enter at a later stage.
There are no replays. When a match is drawn after 90 minutes, the winner will be determined by a penalty shootout. If a match is postponed, this must be rescheduled and played within the postponement window, on or before November 4.