New Look will welcome customers from June 10.
The brand will take over the old Mothercare unit on the Lower Mall – a 5,200sq ft space.
Customers can expect womenswear, 915girls fashion, accessories and shoes.
A New Look Spokesperson said: “We are excited to be opening our brand new store in Churchill Square, Brighton on 10th June.
“It’s a great location, right in the heart of Brighton, and we look forward to inviting our new and loyal customers to shop our latest ranges.”
Mark Buchanan-Smith, centre director at Churchill Square, said: “We’re delighted that New Look has chosen Churchill Square for its new Brighton store, we’re sure it’ll be very well received by our customers.
“Since we reopened in April it has been great to see the shoppers breathing life back into the city.
“We’re all geared up for a very positive summer and things are looking very promising for the rest of 2021.”