Former England captain Sir Ian and Australian spinner Warne took on the tenth hole of the Waterfall course, in aid of Beefy’s Charity Foundation.
Around 80 spectators watched the cricketing icons as part of their attempt to play one hole in each of the first-class 18 cricketing counties across the UK.
The pair arrived by helicopter just after 2pm and played the tricky par three in front of a crowd of club members and the public, with Warne in his usual chirpy spirits despite being six holes down upon arrival.
Botham, who revealed he had played the course before in a charity day year ago, said: "It's fantastic, we have had great reception all the way around.
"The public have been brilliant as they always are in supporting us and helping us to achieve out goal of making as much money as we can.
"I am winning. Winning is important especially when playing Warney it's extremely important, he's Austrialian and I am English, so there is no such thing as a friendly.
"The total is on-going on the website so hopefully we have raised lots and lots when we get in."
Botham's England team won the whole leaving the Aussie a mountain to climb as they flew on to the Ageas Bowl.
The challenge has been filmed and shown on Sky Sports throughout the day as they aim to raise money for the Beefy Charity Foundation.
They teed-off the day at Rockliffe Hall, County Durham at 6am and aim to finish at The Worsley Marriott, Lancashire at 8.30pm.
Proceeds from Mannings Heath also went towards a donation to the golf club’s captain’s charity which is Cardiac Risk in the Young.