A 2-0 defeat at Braintree leaves them unable to finish in the top seven and be part of the end-of-season promotion knockouts.
The best Danny Bloor’s side can hope for is an eighth-placed finish – but it has still been a fine season for the team from Priory Lane, whose campaign ends at home to Concord Rangers next Saturday.
See 42 pictures from the defeat at Braintree – by Lydia and Nick Redman – on this page and the ones linked and get the latest from the Lane in the Eastbourne Herald on Friday.
25. Braintree v Eastbourne Borough pictures by Lydia and Nick Redman (18).jpg
Action from Eastbourne Borough's defeat at Braintree in the National Lague South Photo: Lydia and Nick Redman
26. Braintree v Eastbourne Borough pictures by Lydia and Nick Redman (33).jpg
Action from Eastbourne Borough's defeat at Braintree in the National Lague South Photo: Lydia and Nick Redman
27. Braintree v Eastbourne Borough pictures by Lydia and Nick Redman (31).jpg
Action from Eastbourne Borough's defeat at Braintree in the National Lague South Photo: Lydia and Nick Redman
28. Braintree v Eastbourne Borough pictures by Lydia and Nick Redman (22).jpg
Action from Eastbourne Borough's defeat at Braintree in the National Lague South Photo: Lydia and Nick Redman