The Seagulls, who are seventh in the Premier League, welcome an Ajax club who are having plenty of trouble themselves.
Yesterday they sacked their manager Maurice Steijn in the wake of a 4-3 defeat at Utrecht that left the Dutch giants sitting one point off the bottom of the table with just one win from their first seven games.
Ajax have also been hit by crowd trouble this season with their derby against Feyenoord forced to finish behind closed doors, while their loss at Utrecht was also temporarily suspended late on.
Brighton have had no such trouble but the injuries are concern for head coach De Zerbi, ahead of a tricky fixture schedule in the Premier League and Europa League, where they face Ajax, Fulham, Everton, Ajax again and Sheffield United before mid-November.
Here's the full list of Albion players who have been ruled out of Thursday's clash against Ajax...
5. Pervis Estupinan - muscular
Injured during the first half at Aston Villa and could be second half of November before the Ecuadorian is seen again Photo: Steve Bardens
6. Jakub Moder - knee
Has not played for more than a year with a serious ACL injury. Spotted in training this week and believed to be getting closer to a return but Ajax is too soon. Photo: Warren Little
7. Julio Enciso - knee
Injured his knee earlier this season at Wolves and could return in December. Photo: Clive Mason
8. Adam Lallana
He was on the bench at Man City and could be fit to feature against Ajax. Has not played since coming on as a late sub at Chelsea Photo: GLYN KIRK