The Spanish outfit finished eighth in La Liga last term and will likely provide a stiff test for the Seagulls ahead of their Premier League opener at Everton on August 17.
It will give new head coach Fabian Hurzeler a chance to make his mind up on the fitness of some of his key players – many of which have not yet featured in the pre-season matches in Japan or in the 1-0 win against QPR at Loftus Road last week.
Here's the latest injury and team news ahead of the Villarreal match at the Amex Stadium on August 10 (3pm).
9. Evan Ferguson - out
The Ireland international has not featured in pre-season but has been spotted in training. Struggled with foot and ankle issue last term. Photo: Mike Hewitt
10. Julio Enciso - out
The Paraguay playmaker injured his left knee during the Olympics and is expected to miss the next two to three weeks. Photo: Carl Recine
11. Valentin Barco - doubt
The Argentina ace featured in Japan but missed the game at QPR with minor knock. Hurzeler said: "He felt a little bit, his muscle, but nothing bad." Photo: Masashi Hara
12. Carl Rushworth - out
The goalkeeper has not featured in pre-season due to a minor injury. Has been spotted in full training and could get minutes against Villarreal. Photo: Bryn Lennon